![]() Future studies should assess the impact of PM-guided UTI management on clinical outcomes. Quidel Molecular Programs extend the continuum of diagnostic choices from rapid immunoassays to confirmatory nucleic acid testing. This PM can simultaneously quantify and phenotypically classify the organisms readily through bioinformatic platforms like Explify®, essentially providing dissected and quantitative results for timely and accurate empiric UTI treatment.PM offers potential for building effective diagnostic models beyond usual care testing in complex UTI diseases. All identified organisms were classified into phenotypic groups (0 to 3) with increasing pathogenic potential and clinical relevance. UTI Molecular testing Within a day of sample receipt, if not sooner, our real-time PCR test reports on the identity of the infection causing organism/s. Next, we phenotypically classified the cumulative microbial population using the Explify® data analysis platform for potential pathogenicity.Results revealed 100% positive predictive agreement (PPA) with culture results, which only identified 13 different microorganisms, compared to 19 and 62 organisms identified by PCR and PM, respectively. ‘Multi-Center Clinical Trial Using a Novel Semi-Quantitative Multiplex-PCR Based Diagnostic Panel for Urinary Tract Specimens. We collected 47 urine samples of clinically suspected UTI and in parallel tested each sample by microbial culture, PCR, and PM then we comparatively analyzed the results. However, clinically relevant information is often buried in the enormous amount of sequencing data.Precision metagenomics (PM) is a hybridization capture-based method with potential to enhanced discovery power and better diagnostic yield without diluting clinically relevant information. mNGS is a hypothesis-free approach with potential of deciphering the urobiome. Although PCR testing is fast and highly sensitive, its diagnostic yield is limited to targeted microorganisms. Despite urine culture being the gold standard for UTI diagnosis, some uropathogens may lead to false negative or inconclusive results. Molecular (Nucleic Acid) Based Techniques for UTI Diagnosis 2.1. Urinary tract infections (UTIs) remain a diagnostic challenge and often promote antibiotic overuse. This review therefore focuses on molecular and imaging UTI diagnostic techniques as modern and effective diagnostic platforms at patients point of care applications, aiming at replacing old-century testing methods, saving health care costs, and valuable diagnosis time. You just subscribed to receive the final version of the article
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